FastQCFastQC Report
Tue 29 Jun 2021
S41F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS41F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22562824
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA376610.16691616262219658No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT327050.14495082707731974No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG321500.1424910286052845No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG305900.13557700046767196No Hit
CTGAGGTCGGAGTGATTGGTTAAAAAAGGAGAGAACGCGCTCTCTTTTTT301720.1337243954923373No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATT244530.1083773910570769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)