FastQCFastQC Report
Tue 29 Jun 2021
S3F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS3F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22059111
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA817440.3705679707582051No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT573660.26005581095267166No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG499450.22641438270109798No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATT477060.21626438164257844No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG407770.18485332432481072No Hit
CCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTAAA394750.1789510012438851No Hit
ACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTGA255160.1156710259085237No Hit
CTCCTTTACATAATCGGCTTCAACACATTATGAACTTATTCTGCATTGTG240350.10895724673582721No Hit
CCATCGGATTAAGCGCCAAGAGCCTCTGGAGGGTTCTACGGAACCGCCAA236570.10724366906717138No Hit
CTCACTCTTAATGCTGCGGACAAACAGAATCCTACCTAACCATATCCTTA228170.10343571869238066No Hit
CTTCGACCTGACGTAGCCTCAGTACTTTAAGTTTTTAAAATACTAAGAAC225220.1020984027869482No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)