FastQCFastQC Report
Tue 29 Jun 2021
S39F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS39F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24529412
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA635280.258987047875424No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT545160.22224747988251817No Hit
CTGAGGTCGGAGTGATTGGTTAAAAAAGGAGAGAACGCGCTCTCTTTTTT446420.18199376324226607No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG433370.17667361940840653No Hit
CTCCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTG378190.1541781759791062No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG377250.15379496255352554No Hit
CTGCCCACCTTTTAGGTTACTGACTAGCTCCTTTACATAATCGGCTTCAA372990.1520582719227024No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATT372950.1520419649684224No Hit
CTTCGACCTGACGTAGCCTCAGTACTTTAAGTTTTTAAAATACTAAGAAC312340.12733285249560813No Hit
ACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTGA289430.11799304443172139No Hit
CCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTAAA276240.11261582625788176No Hit
CCACCGTATGCTTTACATTTTAAATCACTGATTAACTGCCTCTAAGCTGC269810.10999448335736707No Hit
CTACGGAACCGCCAAGTTCTTCGAGTTTTAAGCAGTTGCTCGTAGTACTC255000.10395683353518624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)