FastQCFastQC Report
Tue 29 Jun 2021
S35F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS35F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20255295
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA473980.23400301007711813No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT404850.1998736626645033No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATT359960.17771155641031147No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG330600.1632165811458189No Hit
CTCCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTG290640.14348840636485424No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG282920.13967705728304625No Hit
CCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTAAA272100.13433524419170395No Hit
CTTCGACCTGACGTAGCCTCAGTACTTTAAGTTTTTAAAATACTAAGAAC209700.10352848477398133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)