FastQCFastQC Report
Tue 29 Jun 2021
S23M_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS23M_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40458949
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTAGA4081201.0087261535142695Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGT3253300.8040989893237217Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGT2735910.6762187519997122Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGT2214530.5473523298887473Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTA1660650.4104530743000764Illumina Single End PCR Primer 1 (96% over 33bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTAGA1241280.3067998627448281Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGT1225550.3029119713416184Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTG811710.2006255772981152Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTAGATC795310.1965720859432112Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTAG787520.19464667754963183Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTA703420.17386017615039878Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTAGA672860.16630684104028506Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTG658200.16268341523157212Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGT613130.15154372892879645Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTG542420.13406675492237824Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTA521820.12897517431804767Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTG435340.1076004223441395Illumina Single End PCR Primer 1 (96% over 33bp)
GAGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGT408350.1009294630960384Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)