FastQCFastQC Report
Tue 29 Jun 2021
S23M_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS23M_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40458949
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATC3407010.842090584211666TruSeq Adapter, Index 15 (97% over 41bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCG2893630.7152014749567518TruSeq Adapter, Index 15 (97% over 41bp)
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATC2848500.7040469588075557TruSeq Adapter, Index 15 (97% over 41bp)
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATC2316630.5725877852140945TruSeq Adapter, Index 15 (97% over 41bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCGCG1358270.3357155916234996TruSeq Adapter, Index 15 (97% over 41bp)
CCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATC1283300.31718569852123446TruSeq Adapter, Index 15 (97% over 41bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCT1132670.27995536908286967TruSeq Adapter, Index 15 (97% over 41bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCG892230.22052723119426557TruSeq Adapter, Index 15 (97% over 41bp)
CTCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTAT853530.21096198025312027TruSeq Adapter, Index 15 (97% over 41bp)
TCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTAT690800.17074096511997877TruSeq Adapter, Index 15 (97% over 41bp)
TCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATC642720.158857314855114TruSeq Adapter, Index 15 (97% over 41bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCG605780.14972707274229985TruSeq Adapter, Index 15 (97% over 41bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTAT572640.1415360542361098TruSeq Adapter, Index 15 (97% over 41bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTC547860.1354113276644927TruSeq Adapter, Index 15 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCGTA512160.12658756904436644TruSeq Adapter, Index 15 (97% over 41bp)
TCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCT480530.11876976833975594TruSeq Adapter, Index 15 (97% over 41bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCG470410.11626846757685179TruSeq Adapter, Index 15 (97% over 41bp)
CCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTAT456050.11271919100024076TruSeq Adapter, Index 15 (97% over 41bp)
CCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATC427300.10561322292380854TruSeq Adapter, Index 15 (97% over 41bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCGCG405820.10030413790531237TruSeq Adapter, Index 15 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)