FastQCFastQC Report
Tue 29 Jun 2021
S22F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS22F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28462926
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA586320.20599428182471471No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT498070.1749890366155609No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATT446060.1567161436600018No Hit
CCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTGTA376670.13233706190291186No Hit
CTCCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTG359400.12626951986594773No Hit
CCCGCCGCTACAACTGCCCACCTTTTAGGTTACTGACTAGCTCCTTTACA353790.12429853487304852No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG346710.12181108857184957No Hit
CCGCCGCTACAACTGCCCACCTTTTAGGTTACTGACTAGCTCCTTTACAT340800.11973470331195044No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG334990.1176934514743846No Hit
CCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTAAA330800.11622136107861855No Hit
CTGAGGTCGGAGTGATTGGTTAAAAAAGGAGAGAACGCGCTCTCTTTTTT296120.10403709021342358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)