FastQCFastQC Report
Tue 29 Jun 2021
S19F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS19F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24330509
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA775630.31878905615990194No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT605740.2489631433522414No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG513490.2110477836694662No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATT507100.20842145143778126No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG355560.14613750990577304No Hit
CCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTGTA299910.1232649921133997No Hit
CCCGCCGCTACAACTGCCCACCTTTTAGGTTACTGACTAGCTCCTTTACA295320.12137847177796403No Hit
CTTCGACCTGACGTAGCCTCAGTACTTTAAGTTTTTAAAATACTAAGAAC289600.11902751397432747No Hit
CTCCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTG282650.11617101804158722No Hit
CTACGGAACCGCCAAGTTCTTCGAGTTTTAAGCAGTTGCTCGTAGTACTC279110.11471605464563031No Hit
CCATCGGATTAAGCGCCAAGAGCCTCTGGAGGGTTCTACGGAACCGCCAA273020.11221302439665361No Hit
TCCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATT267120.10978808540339209No Hit
CCGCCGCTACAACTGCCCACCTTTTAGGTTACTGACTAGCTCCTTTACAT266320.1094592801161702No Hit
CCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTAAA256880.10557937772695178No Hit
CCGGGTCTATCCCCATAACTTCGCAGATTCATCGTCACGCAAGTCACGGT247710.10181044712217076No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)