FastQCFastQC Report
Tue 29 Jun 2021
S16F_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS16F_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24191000
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTCGTGTGTGTAGATC358210.14807573064362778Illumina Single End PCR Primer 1 (97% over 34bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTCGTGTGTGTAGA258670.10692819643669134Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)