FastQCFastQC Report
Tue 29 Jun 2021
S13M_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS13M_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29537018
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTAGA8684512.940212177139886Illumina Single End PCR Primer 1 (97% over 36bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTAGA4804841.626718039038335Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT3435851.1632352324801374Illumina Single End PCR Primer 1 (97% over 36bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTAGA2789640.9444555303450064Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT2713410.9186472378491288Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTA2050690.6942779396349354Illumina Single End PCR Primer 1 (97% over 36bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT1977990.6696647576271917Illumina Single End PCR Primer 1 (97% over 36bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTAG1352710.45797107886788024Illumina Single End PCR Primer 1 (97% over 36bp)
GGAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT1343960.4550086945134407Illumina Single End PCR Primer 1 (97% over 36bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTAGATC1203250.4073701685119331Illumina Single End PCR Primer 1 (97% over 36bp)
GGAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT861370.29162388701527014Illumina Single End PCR Primer 1 (97% over 36bp)
GGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTA838070.2837354806771625Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT722250.24452366857074062Illumina Single End PCR Primer 1 (97% over 36bp)
GGGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT707240.23944190980958202Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTG511200.17307095794165817Illumina Single End PCR Primer 1 (97% over 36bp)
GGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTA479950.16249101381865969Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTG450350.15246969074535552Illumina Single End PCR Primer 1 (97% over 36bp)
GGAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT434390.14706630168285778Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTG395490.13389638723854927Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTG387940.13134027273843282Illumina Single End PCR Primer 1 (97% over 36bp)
GAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT375760.12721663371705294Illumina Single End PCR Primer 1 (97% over 36bp)
GGAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTG356070.12055042252403407Illumina Single End PCR Primer 1 (97% over 36bp)
GGGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT332750.11265524502168771Illumina Single End PCR Primer 1 (97% over 36bp)
GAGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGT298870.1011848928012977Illumina Single End PCR Primer 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)