FastQCFastQC Report
Tue 29 Jun 2021
S13M_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS13M_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29537018
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCG5307661.7969518791639698TruSeq Adapter, Index 7 (97% over 36bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC3467991.1741164934117587TruSeq Adapter, Index 7 (97% over 36bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCGCG3331281.1278322002579948TruSeq Adapter, Index 7 (97% over 36bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCG2865590.9701690265415419TruSeq Adapter, Index 7 (97% over 36bp)
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC2733530.9254590290732803TruSeq Adapter, Index 7 (97% over 36bp)
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC1991240.6741506539353431TruSeq Adapter, Index 7 (97% over 36bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCGCG1895650.6417878744563855TruSeq Adapter, Index 7 (97% over 36bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCG1726350.584469969175629TruSeq Adapter, Index 7 (97% over 36bp)
CCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC1355180.4588073176513621TruSeq Adapter, Index 7 (97% over 36bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCT1203280.4073803252582911TruSeq Adapter, Index 7 (97% over 36bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCGCG1052480.3563257468983497TruSeq Adapter, Index 7 (97% over 36bp)
TCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC867430.293675549779602TruSeq Adapter, Index 7 (97% over 36bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCG859720.2910652659655758TruSeq Adapter, Index 7 (97% over 36bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTC806190.27294224488064434TruSeq Adapter, Index 7 (97% over 36bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC753250.2550189731407551TruSeq Adapter, Index 7 (97% over 36bp)
ATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC718300.2431863636335936TruSeq Adapter, Index 7 (97% over 36bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCGC543560.18402670167990554TruSeq Adapter, Index 7 (97% over 36bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAAT519300.17581327945833936TruSeq Adapter, Index 7 (97% over 36bp)
TCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCT493090.16693966872349808TruSeq Adapter, Index 7 (97% over 36bp)
CTCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAAT458050.15507658897726237TruSeq Adapter, Index 7 (97% over 36bp)
CTTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC438970.14861689829352442TruSeq Adapter, Index 7 (97% over 36bp)
TCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAAT403980.13677074645788548TruSeq Adapter, Index 7 (97% over 36bp)
ATCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAAT396910.13437713989949832TruSeq Adapter, Index 7 (97% over 36bp)
CTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC379780.12857763772903547TruSeq Adapter, Index 7 (97% over 36bp)
CTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAAT362180.12261901319896273TruSeq Adapter, Index 7 (97% over 36bp)
TCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCG346100.11717499715103265TruSeq Adapter, Index 7 (97% over 36bp)
TTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC330610.11193073044814476TruSeq Adapter, Index 7 (97% over 36bp)
CCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATC300250.1016521031337693TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)