FastQCFastQC Report
Tue 29 Jun 2021
S12M_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS12M_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34273621
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGTAGA3883651.1331309288855123Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGT2013680.5875305676047476Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGT1823530.5320505819913222Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGT1512450.4412869010834893Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGTA1156770.3375102969131858Illumina Single End PCR Primer 1 (96% over 33bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGTAGA901510.26303319395403246Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGTAGATC854800.24940463687802347Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGT828880.241841969367637Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGTAGA695580.2029490843701633Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGTAG571900.1668630227310969Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGT449900.13126713398622225Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTGTA445330.12993374700618882Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTG437230.12757041340919303Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATATGTG365260.10657175674551574Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)