FastQCFastQC Report
Tue 29 Jun 2021
S12M_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS12M_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34273621
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCTCG2674430.7803173175078292TruSeq Adapter, Index 3 (97% over 36bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATC2102900.6135622495212864TruSeq Adapter, Index 3 (97% over 36bp)
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATC1899180.5541229507089432TruSeq Adapter, Index 3 (97% over 36bp)
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATC1578380.4605232694847154TruSeq Adapter, Index 3 (97% over 36bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCGCG972150.28364379707647464TruSeq Adapter, Index 3 (97% over 36bp)
CCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATC862720.2517154519506416TruSeq Adapter, Index 3 (97% over 36bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCT849360.24781741036349791TruSeq Adapter, Index 3 (97% over 36bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCTCG632480.18453842387998629TruSeq Adapter, Index 3 (97% over 36bp)
TCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATC469880.13709669019214515TruSeq Adapter, Index 3 (97% over 36bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCTCG464650.13557073528939353TruSeq Adapter, Index 3 (97% over 36bp)
CTCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGAT460760.13443575162367583TruSeq Adapter, Index 3 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCTCGTA450460.1314305249509528TruSeq Adapter, Index 3 (97% over 36bp)
TCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGAT385020.11233712364386594TruSeq Adapter, Index 3 (97% over 36bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCTC384530.11219415654972668TruSeq Adapter, Index 3 (97% over 36bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCG358810.1046898429553154TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)