FastQCFastQC Report
Tue 29 Jun 2021
29F_R1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename29F_R1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137641794
Sequences flagged as poor quality0
Sequence length20-136
%GC19

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACCCATCATTAGATCTCGTATGCCGTCTTCTGCTTGAAA8551170.6212626086521366TruSeq Adapter, Index 9 (96% over 29bp)
GAGCCCACGAGACCCATCATTAGATCGCGTATGCCGTCTTCTGCTTGAAA3239140.23533113786645357Illumina PCR Primer Index 9 (96% over 26bp)
CCCACGAGACCCATCATTAGATCTCGTATGCCGTCTTCTGCTTGAAAAGG1463410.10632017772160104TruSeq Adapter, Index 9 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)