FastQCFastQC Report
Tue 29 Jun 2021
22F_R1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename22F_R1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125853047
Sequences flagged as poor quality0
Sequence length20-136
%GC19

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACAGAGGCAACCATCTCGTATGCCGTCTTCTGCTTGAAA5262060.4181114502535644Illumina PCR Primer Index 1 (96% over 27bp)
GAGCCCACGAGACAGAGGCAACCATCGCGTATGCCGTCTTCTGCTTGAAA2333940.18544962205007243RNA PCR Primer, Index 14 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)