FastQCFastQC Report
Tue 29 Jun 2021
19F_R1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename19F_R1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199653889
Sequences flagged as poor quality0
Sequence length20-136
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGAGAATGGTTATCTCGTATGCCGTCTTCTGCTTGAAA26796731.34215918028023Illumina PCR Primer Index 10 (96% over 28bp)
GAGCCCACGAGACGAGAATGGTTATCGCGTATGCCGTCTTCTGCTTGAAA4408410.2208026110625874TruSeq Adapter, Index 10 (96% over 26bp)
CCCACGAGACGAGAATGGTTATCTCGTATGCCGTCTTCTGCTTGAAAAGG3944920.19758793679195502Illumina PCR Primer Index 10 (96% over 28bp)
CCGAGCCCACGAGACGAGAATGGTTATCTCGTATGCCGTCTTCTGCTTGA2544950.1274680905414269Illumina PCR Primer Index 10 (96% over 28bp)
CACGAGACGAGAATGGTTATCTCGTATGCCGTCTTCTGCTTGAAAAGGGG2484670.12444886560661987RNA PCR Primer, Index 31 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)