FastQCFastQC Report
Tue 29 Jun 2021
16F_R1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16F_R1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184170994
Sequences flagged as poor quality0
Sequence length20-136
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACCGGTTACGGCATCTCGTATGCCGTCTTCTGCTTGAAA16196120.8794066670455175RNA PCR Primer, Index 34 (96% over 29bp)
GAGCCCACGAGACCGGTTACGGCATCGCGTATGCCGTCTTCTGCTTGAAA5186410.28160840571887236Illumina PCR Primer Index 3 (96% over 27bp)
CCCACGAGACCGGTTACGGCATCTCGTATGCCGTCTTCTGCTTGAAAAGG2364030.12836060384188402RNA PCR Primer, Index 34 (96% over 29bp)
GAGCCCACGAGACCGGTTACGGCATCTCGTATGCCGTCTTCTGCGTGAAA1887570.10249008049552036Illumina PCR Primer Index 3 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)