FastQCFastQC Report
Sun 6 Dec 2020
zr3644_3_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr3644_3_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39620531
Sequences flagged as poor quality0
Sequence length20-135
%GC18

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACCTCTGCAGCGATCTCGTATGCCGTCTTCTGCTTGAAA1027990.2594589153789988TruSeq Adapter, Index 5 (96% over 29bp)
GAGCCCACGAGACCTCTGCAGCGATCGCGTATGCCGTCTTCTGCTTGAAA523440.13211332276187818RNA PCR Primer, Index 33 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)