FastQCFastQC Report
Tue 10 Nov 2020
zr3534_7_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr3534_7_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67258007
Sequences flagged as poor quality0
Sequence length20-135
%GC18

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACTTCCTACAGCATCTCGTATGCCGTCTTCTGCTTGAAA1919160.28534297782567364RNA PCR Primer, Index 43 (96% over 32bp)
GAGCCCACGAGACTTCCTACAGCATCGCGTATGCCGTCTTCTGCTTGAAA710160.10558742842320618RNA PCR Primer, Index 24 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)