FastQCFastQC Report
Tue 10 Nov 2020
zr3534_5_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr3534_5_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85730862
Sequences flagged as poor quality0
Sequence length20-135
%GC18

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACAATGCGAACAATCTCGTATGCCGTCTTCTGCTTGAAA2834730.3306545547156635TruSeq Adapter, Index 4 (96% over 29bp)
GAGCCCACGAGACAATGCGAACAATCGCGTATGCCGTCTTCTGCTTGAAA1300270.15166883543058274RNA PCR Primer, Index 36 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)