sending incremental file list Pool26_16_P_31_1.fastq.gz Pool26_16_P_31_2.fastq.gz Pool26_8_P_31_1.fastq.gz Pool26_8_P_31_2.fastq.gz Pool32_16_P_31_1.fastq.gz Pool32_16_P_31_2.fastq.gz Pool32_8_P_31_1.fastq.gz Pool32_8_P_31_2.fastq.gz sent 15,444,343,700 bytes received 168 bytes 363,396,326.31 bytes/sec total size is 15,440,573,460 speedup is 1.00 Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 27 Read1 before filtering: total reads: 25500194 total bases: 3850529294 Q20 bases: 3771681008(97.9523%) Q30 bases: 3633368990(94.3602%) Read2 before filtering: total reads: 25500194 total bases: 3850529294 Q20 bases: 3736064413(97.0273%) Q30 bases: 3558900261(92.4263%) Read1 after filtering: total reads: 25291362 total bases: 3657408684 Q20 bases: 3589571176(98.1452%) Q30 bases: 3461781287(94.6512%) Read2 aftering filtering: total reads: 25291362 total bases: 3656885662 Q20 bases: 3570619192(97.641%) Q30 bases: 3410451310(93.2611%) Filtering result: reads passed filter: 50582724 reads failed due to low quality: 354586 reads failed due to too many N: 0 reads failed due to too short: 63078 reads with adapter trimmed: 13670017 bases trimmed due to adapters: 319086910 Duplication rate: 9.37616% Insert size peak (evaluated by paired-end reads): 151 JSON report: Pool26_16_P_31_1.fastp-trim.20201029.report.json HTML report: Pool26_16_P_31_1.fastp-trim.20201029.report.html /gscratch/srlab/programs/fastp-0.20.0/fastp --in1 Pool26_16_P_31_1.fastq.gz --in2 Pool26_16_P_31_2.fastq.gz --detect_adapter_for_pe --thread 27 --html Pool26_16_P_31_1.fastp-trim.20201029.report.html --json Pool26_16_P_31_1.fastp-trim.20201029.report.json --out1 Pool26_16_P_31_1.fastp-trim.20201029.fq.gz --out2 Pool26_16_P_31_2.fastp-trim.20201029.fq.gz fastp v0.20.0, time used: 229 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 27 Read1 before filtering: total reads: 28254350 total bases: 4266406850 Q20 bases: 4183965157(98.0677%) Q30 bases: 4039752328(94.6875%) Read2 before filtering: total reads: 28254350 total bases: 4266406850 Q20 bases: 4155778410(97.407%) Q30 bases: 3982295949(93.3407%) Read1 after filtering: total reads: 28055070 total bases: 4078248589 Q20 bases: 4008391055(98.2871%) Q30 bases: 3874467035(95.0032%) Read2 aftering filtering: total reads: 28055070 total bases: 4077615781 Q20 bases: 3995707789(97.9913%) Q30 bases: 3838112983(94.1264%) Filtering result: reads passed filter: 56110140 reads failed due to low quality: 363760 reads failed due to too many N: 0 reads failed due to too short: 34800 reads with adapter trimmed: 13445462 bases trimmed due to adapters: 313346195 Duplication rate: 8.98957% Insert size peak (evaluated by paired-end reads): 166 JSON report: Pool26_8_P_31_1.fastp-trim.20201029.report.json HTML report: Pool26_8_P_31_1.fastp-trim.20201029.report.html /gscratch/srlab/programs/fastp-0.20.0/fastp --in1 Pool26_8_P_31_1.fastq.gz --in2 Pool26_8_P_31_2.fastq.gz --detect_adapter_for_pe --thread 27 --html Pool26_8_P_31_1.fastp-trim.20201029.report.html --json Pool26_8_P_31_1.fastp-trim.20201029.report.json --out1 Pool26_8_P_31_1.fastp-trim.20201029.fq.gz --out2 Pool26_8_P_31_2.fastp-trim.20201029.fq.gz fastp v0.20.0, time used: 300 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 27 Read1 before filtering: total reads: 20493385 total bases: 3094501135 Q20 bases: 3028224208(97.8582%) Q30 bases: 2915807518(94.2254%) Read2 before filtering: total reads: 20493385 total bases: 3094501135 Q20 bases: 3002548385(97.0285%) Q30 bases: 2859363539(92.4014%) Read1 after filtering: total reads: 20329409 total bases: 2943699896 Q20 bases: 2887058726(98.0759%) Q30 bases: 2782811089(94.5345%) Read2 aftering filtering: total reads: 20329409 total bases: 2943560230 Q20 bases: 2873719143(97.6273%) Q30 bases: 2743776164(93.2128%) Filtering result: reads passed filter: 40658818 reads failed due to low quality: 287516 reads failed due to too many N: 0 reads failed due to too short: 40436 reads with adapter trimmed: 10654425 bases trimmed due to adapters: 247595336 Duplication rate: 8.33341% Insert size peak (evaluated by paired-end reads): 163 JSON report: Pool32_16_P_31_1.fastp-trim.20201029.report.json HTML report: Pool32_16_P_31_1.fastp-trim.20201029.report.html /gscratch/srlab/programs/fastp-0.20.0/fastp --in1 Pool32_16_P_31_1.fastq.gz --in2 Pool32_16_P_31_2.fastq.gz --detect_adapter_for_pe --thread 27 --html Pool32_16_P_31_1.fastp-trim.20201029.report.html --json Pool32_16_P_31_1.fastp-trim.20201029.report.json --out1 Pool32_16_P_31_1.fastp-trim.20201029.fq.gz --out2 Pool32_16_P_31_2.fastp-trim.20201029.fq.gz fastp v0.20.0, time used: 187 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 27 Read1 before filtering: total reads: 27091891 total bases: 4090875541 Q20 bases: 4011891184(98.0693%) Q30 bases: 3869655741(94.5924%) Read2 before filtering: total reads: 27091891 total bases: 4090875541 Q20 bases: 3977411201(97.2264%) Q30 bases: 3798491704(92.8528%) Read1 after filtering: total reads: 26875465 total bases: 3920333152 Q20 bases: 3851190175(98.2363%) Q30 bases: 3717879748(94.8358%) Read2 aftering filtering: total reads: 26875465 total bases: 3919915830 Q20 bases: 3834432333(97.8193%) Q30 bases: 3671217509(93.6555%) Filtering result: reads passed filter: 53750930 reads failed due to low quality: 376754 reads failed due to too many N: 0 reads failed due to too short: 56098 reads with adapter trimmed: 11720476 bases trimmed due to adapters: 272785716 Duplication rate: 10.2548% Insert size peak (evaluated by paired-end reads): 166 JSON report: Pool32_8_P_31_1.fastp-trim.20201029.report.json HTML report: Pool32_8_P_31_1.fastp-trim.20201029.report.html /gscratch/srlab/programs/fastp-0.20.0/fastp --in1 Pool32_8_P_31_1.fastq.gz --in2 Pool32_8_P_31_2.fastq.gz --detect_adapter_for_pe --thread 27 --html Pool32_8_P_31_1.fastp-trim.20201029.report.html --json Pool32_8_P_31_1.fastp-trim.20201029.report.json --out1 Pool32_8_P_31_1.fastp-trim.20201029.fq.gz --out2 Pool32_8_P_31_2.fastp-trim.20201029.fq.gz fastp v0.20.0, time used: 226 seconds [INFO ] multiqc : This is MultiQC v1.8 [INFO ] multiqc : Template : default [INFO ] multiqc : Searching : /gscratch/scrubbed/samwhite/outputs/202001029_ssal_RNAseq_fastp_trimming [INFO ] fastp : Found 4 reports [INFO ] multiqc : Compressing plot data [INFO ] multiqc : Report : multiqc_report.html [INFO ] multiqc : Data : multiqc_data [INFO ] multiqc : MultiQC complete Removing Pool26_16_P_31_1.fastq.gz rm: cannot remove ‘Pool26_16_P_31_1.fastq.gz’: No such file or directory