Version MEGAN Community Edition (version 6.18.0, built 28 Oct 2019) Copyright (C) 2019 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY. This is free software, licensed under the terms of the GNU General Public License, Version 3. Sources available at: https://github.com/danielhuson/megan-ce Java version: 11.0.7 Opening startup files Loading ncbi.map: 2,175,506 Loading ncbi.tre: 2,175,510 Induced tree has 2,175,510 of 2,175,510 nodes Executing: add file='/home/samb/data/metagenomics/Library_Geoduck_MG_1_S3_L002_R1.rma6';add file='/home/samb/data/metagenomics/Library_Geoduck_MG_2_S4_L002_R1.rma6';add file='/home/samb/data/metagenomics/Library_Geoduck_MG_3_S1_L002_R1.rma6';add file='/home/samb/data/metagenomics/Library_Geoduck_MG_5_S6_L002_R1.rma6';add file='/home/samb/data/metagenomics/Library_Geoduck_MG_6_S5_L002_R1.rma6';add file='/home/samb/data/metagenomics/Library_Geoduck_MG_7_S2_L002_R1.rma6'; Loading MEGAN File: Library_Geoduck_MG_1_S3_L002_R1.rma6 Loading MEGAN File: Library_Geoduck_MG_2_S4_L002_R1.rma6 Loading MEGAN File: Library_Geoduck_MG_3_S1_L002_R1.rma6 Loading MEGAN File: Library_Geoduck_MG_5_S6_L002_R1.rma6 Loading MEGAN File: Library_Geoduck_MG_6_S5_L002_R1.rma6 Loading MEGAN File: Library_Geoduck_MG_7_S2_L002_R1.rma6 Induced tree has 2,175,510 of 2,175,510 nodes Executing: set mode=ABSOLUTE; Executing: apply; Selected: 6 Executing: compare mode=ABSOLUTE readAssignmentMode=readCount keep1=false ignoreUnassigned=false meganFile='/home/samb/data/metagenomics/Library_Geoduck_MG_1_S3_L002_R1.rma6', '/home/samb/data/metagenomics/Library_Geoduck_MG_2_S4_L002_R1.rma6', '/home/samb/data/metagenomics/Library_Geoduck_MG_3_S1_L002_R1.rma6', '/home/samb/data/metagenomics/Library_Geoduck_MG_5_S6_L002_R1.rma6', '/home/samb/data/metagenomics/Library_Geoduck_MG_6_S5_L002_R1.rma6', '/home/samb/data/metagenomics/Library_Geoduck_MG_7_S2_L002_R1.rma6'; Executing: open file='/home/samb/data/metagenomics/Library_Geoduck_MG_1_S3_L002_R1.rma6' readOnly=true;update; Loading MEGAN File: Library_Geoduck_MG_1_S3_L002_R1.rma6 Info: Opened file 'Library_Geoduck_MG_1_S3_L002_R1.rma6' with 78,338,406 reads updating viewer Induced tree has 371 of 2,175,510 nodes Induced tree has 371 of 2,175,510 nodes Executing: open file='/home/samb/data/metagenomics/Library_Geoduck_MG_2_S4_L002_R1.rma6' readOnly=true;update; Loading MEGAN File: Library_Geoduck_MG_2_S4_L002_R1.rma6 Opened file '/home/samb/data/metagenomics/Library_Geoduck_MG_2_S4_L002_R1.rma6' with 80,708,846 reads updating viewer Induced tree has 319 of 2,175,510 nodes Induced tree has 319 of 2,175,510 nodes Executing: open file='/home/samb/data/metagenomics/Library_Geoduck_MG_3_S1_L002_R1.rma6' readOnly=true;update; Loading MEGAN File: Library_Geoduck_MG_3_S1_L002_R1.rma6 Opened file '/home/samb/data/metagenomics/Library_Geoduck_MG_3_S1_L002_R1.rma6' with 41,066,115 reads updating viewer Induced tree has 398 of 2,175,510 nodes Induced tree has 398 of 2,175,510 nodes Executing: open file='/home/samb/data/metagenomics/Library_Geoduck_MG_5_S6_L002_R1.rma6' readOnly=true;update; Loading MEGAN File: Library_Geoduck_MG_5_S6_L002_R1.rma6 Opened file '/home/samb/data/metagenomics/Library_Geoduck_MG_5_S6_L002_R1.rma6' with 80,636,117 reads updating viewer Induced tree has 253 of 2,175,510 nodes Induced tree has 253 of 2,175,510 nodes Executing: open file='/home/samb/data/metagenomics/Library_Geoduck_MG_6_S5_L002_R1.rma6' readOnly=true;update; Loading MEGAN File: Library_Geoduck_MG_6_S5_L002_R1.rma6 Opened file '/home/samb/data/metagenomics/Library_Geoduck_MG_6_S5_L002_R1.rma6' with 83,337,226 reads updating viewer Induced tree has 284 of 2,175,510 nodes Induced tree has 284 of 2,175,510 nodes Executing: open file='/home/samb/data/metagenomics/Library_Geoduck_MG_7_S2_L002_R1.rma6' readOnly=true;update; Loading MEGAN File: Library_Geoduck_MG_7_S2_L002_R1.rma6 Opened file '/home/samb/data/metagenomics/Library_Geoduck_MG_7_S2_L002_R1.rma6' with 101,937,343 reads updating viewer Induced tree has 281 of 2,175,510 nodes Induced tree has 281 of 2,175,510 nodes Computing comparison: Total assigned: 466,024,048 Induced tree has 585 of 2,175,510 nodes Induced tree has 585 of 2,175,510 nodes Executing: show window=samplesViewer; Executing: new attribute='pH' position=6; Executing: new attribute='Day' position=6; Executing: show window=samplesViewer; Executing: move sample= 'Library_Geoduck_MG_3_S1_L002_R1' direction=down; Executing: update reinduce=true;select samples name='Library_Geoduck_MG_3_S1_L002_R1'; Selected 1 rows Executing: colorBy attribute='pH'; Induced tree has 585 of 2,175,510 nodes Induced tree has 585 of 2,175,510 nodes Executing: shapeBy attribute='Day'; Executing: set nodeShape=Oval sample= 'Library_Geoduck_MG_2_S4_L002_R1' 'Library_Geoduck_MG_7_S2_L002_R1';set nodeShape=Diamond sample= 'Library_Geoduck_MG_3_S1_L002_R1';set nodeShape=Triangle sample= 'Library_Geoduck_MG_5_S6_L002_R1';set nodeShape=Rectangle sample= 'Library_Geoduck_MG_1_S3_L002_R1' 'Library_Geoduck_MG_6_S5_L002_R1'; Executing: open viewer=INTERPRO2GO; Loading interpro2go.map: 13,501 Loading interpro2go.tre: 29,204 Induced tree has 25,735 of 29,204 nodes Induced tree has 25,735 of 29,204 nodes Executing: uncollapse nodes=selected; Induced tree has 25,735 of 29,204 nodes Induced tree has 25,735 of 29,204 nodes Executing: uncollapse nodes=selected; Induced tree has 25,735 of 29,204 nodes Induced tree has 25,735 of 29,204 nodes Executing: uncollapse nodes=selected; Induced tree has 25,735 of 29,204 nodes Induced tree has 25,735 of 29,204 nodes Executing: export what=CSV format=interpro2goName_to_count separator=tab counts=assigned file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_NameToCount.txt'; Info: Wrote 62 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_NameToCount.txt Executing: export what=CSV format=interpro2goPath_to_count separator=tab counts=assigned file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_interpro2goPathToCount.txt'; Info: Wrote 62 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_interpro2goPathToCount.txt Executing: export what=CSV format=interpro2goName_to_percent separator=tab counts=assigned file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_interpro2goNameToPercent.txt'; Info: Wrote 62 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_interpro2goNameToPercent.txt Executing: export what=CSV format=interpro2goPath_to_percent separator=tab counts=assigned file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_interpro2goPathToPercent.txt'; Info: Wrote 62 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_interpro2goPathToPercent.txt Executing: save file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH.megan' summary=true; done Executing: compareBy attribute='Day' mode=absolute samples='Library_Geoduck_MG_1_S3_L002_R1' 'Library_Geoduck_MG_2_S4_L002_R1' 'Library_Geoduck_MG_5_S6_L002_R1' 'Library_Geoduck_MG_3_S1_L002_R1' 'Library_Geoduck_MG_6_S5_L002_R1' 'Library_Geoduck_MG_7_S2_L002_R1'; Loading seed.map: 13,662 Loading seed.tre: 21,085 Loading eggnog.map: 30,875 Loading eggnog.tre: 30,986 Info: Wrote 463,244,224 reads to file '/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH-Day.megan' Executing: update reprocess=true reinduce=true; Induced tree has 584 of 2,175,510 nodes Induced tree has 584 of 2,175,510 nodes Executing: open viewer=INTERPRO2GO; Induced tree has 25,734 of 29,204 nodes Induced tree has 25,734 of 29,204 nodes Executing: uncollapse nodes=selected; Induced tree has 25,734 of 29,204 nodes Induced tree has 25,734 of 29,204 nodes Executing: uncollapse nodes=selected; Induced tree has 25,734 of 29,204 nodes Induced tree has 25,734 of 29,204 nodes Executing: uncollapse nodes=selected; Induced tree has 25,734 of 29,204 nodes Induced tree has 25,734 of 29,204 nodes Executing: export what=CSV format=interpro2goName_to_count separator=tab counts=assigned file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_interpro2goNameToCount.txt'; Info: Wrote 62 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_interpro2goNameToCount.txt Executing: export what=CSV format=interpro2goPath_to_count separator=tab counts=assigned file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_interpro2goPathToCount.txt'; Info: Wrote 62 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_interpro2goPathToCount.txt Executing: export what=CSV format=interpro2goName_to_percent separator=tab counts=assigned file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_interpro2goNameToPercent.txt'; Info: Wrote 62 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_interpro2goNameToPercent.txt Executing: export what=CSV format=interpro2goPath_to_percent separator=tab counts=assigned file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_interpro2goPathToPercent.txt'; Info: Wrote 62 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_interpro2goPathToPercent.txt Executing: show window=mainViewer; Executing: collapse rank='Class';select rank='Class'; Induced tree has 584 of 2,175,510 nodes Induced tree has 584 of 2,175,510 nodes Executing: collapse nodes=selected; Induced tree has 584 of 2,175,510 nodes Induced tree has 584 of 2,175,510 nodes Executing: collapse nodes=top; Induced tree has 584 of 2,175,510 nodes Induced tree has 584 of 2,175,510 nodes Executing: uncollapse nodes=selected; Induced tree has 584 of 2,175,510 nodes Induced tree has 584 of 2,175,510 nodes Executing: collapse rank='Class';select rank='Class'; Induced tree has 584 of 2,175,510 nodes Induced tree has 584 of 2,175,510 nodes Executing: export what=CSV format=taxonName_to_count separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_readsNameToCount.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_readsNameToCount.txt Executing: export what=CSV format=taxonRank_to_count separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_readsTaxonRankToCount.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_readsTaxonRankToCount.txt Executing: export what=CSV format=taxonPath_to_count separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_readsTaxonPathToCount.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_readsTaxonPathToCount.txt Executing: export what=CSV format=taxonPath_to_percent separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_readsTaxonPathToPercent.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_day_readsTaxonPathToPercent.txt Executing: close what=current; Executing: save file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_Day.megan' summary=true; done Executing: show window=mainViewer; Executing: uncollapse nodes=selected; Induced tree has 585 of 2,175,510 nodes Induced tree has 585 of 2,175,510 nodes Executing: collapse rank='Class';select rank='Class'; Induced tree has 585 of 2,175,510 nodes Induced tree has 585 of 2,175,510 nodes Executing: show window=mainViewer; Executing: close what=current; Executing: collapse rank='Class';select rank='Class'; Induced tree has 585 of 2,175,510 nodes Induced tree has 585 of 2,175,510 nodes Using experimental comparison data access Executing: export what=CSV format=taxonName_to_count separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsNameToCount.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsNameToCount.txt Executing: export what=CSV format=taxonRank_to_count separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToCount.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToCount.txt Executing: export what=CSV format=taxonPath_to_count separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToCount.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToCount.txt Executing: export what=CSV format=taxonRank_to_count separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonRankToCount.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonRankToCount.txt Executing: export what=CSV format=taxonPath_to_count separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToCount.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToCount.txt Executing: export what=CSV format=taxonPath_to_percent separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToPercent.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToPercent.txt Executing: close what=current; Executing: quit; Executing: save file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH.megan' summary=true; done Induced tree has 2,175,510 of 2,175,510 nodes Executing: open file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH.megan'; Loading MEGAN File: abs-reads_abs-comparison_pH.megan Info: Opened file 'abs-reads_abs-comparison_pH.megan' with 466,024,064 reads Induced tree has 585 of 2,175,510 nodes Induced tree has 585 of 2,175,510 nodes Executing: show window=samplesViewer; Executing: compareBy attribute='pH' mode=absolute samples='Library_Geoduck_MG_1_S3_L002_R1' 'Library_Geoduck_MG_2_S4_L002_R1' 'Library_Geoduck_MG_5_S6_L002_R1' 'Library_Geoduck_MG_3_S1_L002_R1' 'Library_Geoduck_MG_6_S5_L002_R1' 'Library_Geoduck_MG_7_S2_L002_R1'; Info: Wrote 463,244,160 reads to file '/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH-pH.megan' Executing: update reprocess=true reinduce=true; Induced tree has 584 of 2,175,510 nodes Induced tree has 584 of 2,175,510 nodes Executing: uncollapse nodes=selected; Induced tree has 584 of 2,175,510 nodes Induced tree has 584 of 2,175,510 nodes Executing: collapse rank='Class';select rank='Class'; Induced tree has 584 of 2,175,510 nodes Induced tree has 584 of 2,175,510 nodes Save canceled Executing: export what=CSV format=taxonPath_to_count separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToCount.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToCount.txt Executing: export what=CSV format=taxonPath_to_percent separator=tab counts=summarized file='/home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToPercent.txt'; Info: Wrote 26 line(s) to file: /home/samb/analyses/20200618_metagenomics_megan_tables/abs-reads_abs-comparison_pH_readsTaxonPathToPercent.txt Executing: close what=current;