WARNING An augustus species is mentioned in the config file, dataset default species (fly) will be ignored INFO ****************** Start a BUSCO 3.0.2 analysis, current time: 06/15/2020 20:30:51 ****************** INFO Configuration loaded from /gscratch/scrubbed/samwhite/outputs/20200527_cbai_busco_transcriptome_v1.7/config.ini INFO Init tools... INFO Check dependencies... INFO Check input file... INFO To reproduce this run: python /gscratch/srlab/programs/busco-v3/scripts/run_BUSCO.py -i /gscratch/srlab/sam/data/C_bairdi/transcriptomes/cbai_transcriptome_v1.7.fasta -o cbai_transcriptome_v1.7.fasta -l /gscratch/srlab/sam/data/databases/BUSCO/metazoa_odb9/ -m transcriptome -c 28 --long -z INFO Mode is: transcriptome INFO The lineage dataset is: metazoa_odb9 (eukaryota) INFO Temp directory is ./tmp/ INFO ****** Step 1/2, current time: 06/15/2020 20:30:51 ****** INFO Create blast database... INFO [makeblastdb] Building a new DB, current time: 06/15/2020 20:30:51 INFO [makeblastdb] New DB name: /gscratch/scrubbed/samwhite/outputs/20200527_cbai_busco_transcriptome_v1.7/tmp/cbai_transcriptome_v1.7.fasta_2569186367 INFO [makeblastdb] New DB title: /gscratch/srlab/sam/data/C_bairdi/transcriptomes/cbai_transcriptome_v1.7.fasta INFO [makeblastdb] Sequence type: Nucleotide INFO [makeblastdb] Keep MBits: T INFO [makeblastdb] Maximum file size: 1000000000B INFO [makeblastdb] Adding sequences from FASTA; added 20526 sequences in 0.518939 seconds. INFO [makeblastdb] 1 of 1 task(s) completed at 06/15/2020 20:30:52 INFO Running tblastn, writing output to /gscratch/scrubbed/samwhite/outputs/20200527_cbai_busco_transcriptome_v1.7/run_cbai_transcriptome_v1.7.fasta/blast_output/tblastn_cbai_transcriptome_v1.7.fasta.tsv... INFO [tblastn] 1 of 1 task(s) completed at 06/15/2020 20:31:45 INFO ****** Step 2/2, current time: 06/15/2020 20:31:45 ****** INFO Maximum number of candidate transcript per BUSCO limited to: 3 INFO Getting coordinates for candidate transcripts... INFO Extracting candidate transcripts... INFO Translating candidate transcripts... INFO Running HMMER to confirm transcript orthology: INFO [hmmsearch] 159 of 1581 task(s) completed at 06/15/2020 20:31:53 INFO [hmmsearch] 475 of 1581 task(s) completed at 06/15/2020 20:31:54 INFO [hmmsearch] 1107 of 1581 task(s) completed at 06/15/2020 20:31:56 INFO [hmmsearch] 1581 of 1581 task(s) completed at 06/15/2020 20:31:58 INFO Results: INFO C:86.7%[S:66.5%,D:20.2%],F:8.2%,M:5.1%,n:978 INFO 848 Complete BUSCOs (C) INFO 650 Complete and single-copy BUSCOs (S) INFO 198 Complete and duplicated BUSCOs (D) INFO 80 Fragmented BUSCOs (F) INFO 50 Missing BUSCOs (M) INFO 978 Total BUSCO groups searched INFO BUSCO analysis done with WARNING(s). Total running time: 70.96418237686157 seconds INFO Results written in /gscratch/scrubbed/samwhite/outputs/20200527_cbai_busco_transcriptome_v1.7/run_cbai_transcriptome_v1.7.fasta/