[2020-04-30 07:04:27.267] [jointLog] [info] setting maxHashResizeThreads to 28 [2020-04-30 07:04:27.267] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored. [2020-04-30 07:04:27.267] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65 [2020-04-30 07:04:27.267] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.35. [2020-04-30 07:04:27.267] [jointLog] [info] parsing read library format [2020-04-30 07:04:27.267] [jointLog] [info] There is 1 library. [2020-04-30 07:04:27.347] [jointLog] [info] Loading pufferfish index [2020-04-30 07:04:27.347] [jointLog] [warning] The index did not record if the `--keepDuplicates` flag was used. Please consider re-indexing with a newer version of salmon that will propagate this information. [2020-04-30 07:04:27.347] [jointLog] [info] Loading dense pufferfish index. [2020-04-30 07:04:27.461] [jointLog] [info] done [2020-04-30 07:04:27.461] [jointLog] [info] Index contained 40,435 targets [2020-04-30 07:04:27.477] [jointLog] [info] Number of decoys : 0 [2020-04-30 07:04:28.301] [jointLog] [info] Automatically detected most likely library type as IU [2020-04-30 07:04:28.516] [jointLog] [info] Computed 5,798 rich equivalence classes for further processing [2020-04-30 07:04:28.516] [jointLog] [info] Counted 75,532 total reads in the equivalence classes [2020-04-30 07:04:28.550] [jointLog] [info] Number of mappings discarded because of alignment score : 51,320 [2020-04-30 07:04:28.550] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 2,754 [2020-04-30 07:04:28.550] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0 [2020-04-30 07:04:28.550] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings to valid targets : 331 [2020-04-30 07:04:28.554] [jointLog] [warning] Only 75532 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2020-04-30 07:04:28.554] [jointLog] [info] Mapping rate = 95.9587% [2020-04-30 07:04:28.554] [jointLog] [info] finished quantifyLibrary() [2020-04-30 07:04:28.555] [jointLog] [info] Starting optimizer [2020-04-30 07:04:28.568] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2020-04-30 07:04:28.571] [jointLog] [info] iteration = 0 | max rel diff. = 153.047 [2020-04-30 07:04:28.640] [jointLog] [info] iteration = 100 | max rel diff. = 0.0554974 [2020-04-30 07:04:28.676] [jointLog] [info] iteration = 161 | max rel diff. = 0.00152478 [2020-04-30 07:04:28.677] [jointLog] [info] Finished optimizer [2020-04-30 07:04:28.677] [jointLog] [info] writing output [2020-04-30 07:04:28.517] [fileLog] [info] At end of round 0 ================== Observed 78713 total fragments (78713 in most recent round)