[2020-04-30 07:03:16.762] [jointLog] [info] setting maxHashResizeThreads to 28 [2020-04-30 07:03:16.762] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored. [2020-04-30 07:03:16.762] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65 [2020-04-30 07:03:16.762] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.35. [2020-04-30 07:03:16.762] [jointLog] [info] parsing read library format [2020-04-30 07:03:16.762] [jointLog] [info] There is 1 library. [2020-04-30 07:03:16.854] [jointLog] [info] Loading pufferfish index [2020-04-30 07:03:16.854] [jointLog] [warning] The index did not record if the `--keepDuplicates` flag was used. Please consider re-indexing with a newer version of salmon that will propagate this information. [2020-04-30 07:03:16.854] [jointLog] [info] Loading dense pufferfish index. [2020-04-30 07:03:16.998] [jointLog] [info] done [2020-04-30 07:03:16.998] [jointLog] [info] Index contained 40,435 targets [2020-04-30 07:03:17.014] [jointLog] [info] Number of decoys : 0 [2020-04-30 07:03:17.821] [jointLog] [info] Automatically detected most likely library type as IU [2020-04-30 07:03:26.045] [jointLog] [info] Computed 14,142 rich equivalence classes for further processing [2020-04-30 07:03:26.045] [jointLog] [info] Counted 2,610,801 total reads in the equivalence classes [2020-04-30 07:03:26.057] [jointLog] [info] Number of mappings discarded because of alignment score : 828,701 [2020-04-30 07:03:26.057] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 20,911 [2020-04-30 07:03:26.057] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0 [2020-04-30 07:03:26.057] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings to valid targets : 4,619 [2020-04-30 07:03:26.060] [jointLog] [warning] Only 2610801 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2020-04-30 07:03:26.060] [jointLog] [info] Mapping rate = 99.1818% [2020-04-30 07:03:26.060] [jointLog] [info] finished quantifyLibrary() [2020-04-30 07:03:26.061] [jointLog] [info] Starting optimizer [2020-04-30 07:03:26.075] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2020-04-30 07:03:26.078] [jointLog] [info] iteration = 0 | max rel diff. = 5797.17 [2020-04-30 07:03:26.156] [jointLog] [info] iteration = 100 | max rel diff. = 11.9299 [2020-04-30 07:03:26.223] [jointLog] [info] iteration = 200 | max rel diff. = 3.52464 [2020-04-30 07:03:26.284] [jointLog] [info] iteration = 300 | max rel diff. = 2.25293 [2020-04-30 07:03:26.345] [jointLog] [info] iteration = 400 | max rel diff. = 0.033746 [2020-04-30 07:03:26.379] [jointLog] [info] iteration = 456 | max rel diff. = 0.00325304 [2020-04-30 07:03:26.380] [jointLog] [info] Finished optimizer [2020-04-30 07:03:26.380] [jointLog] [info] writing output [2020-04-30 07:03:26.046] [fileLog] [info] At end of round 0 ================== Observed 2632340 total fragments (2632340 in most recent round)