FastQCFastQC Report
Sun 26 Apr 2020
571_S76_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename571_S76_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401824
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29511321.052072157417765No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90050.6423773597826832No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70270.5012754810874974No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAAATCTCGTATGCCGT42160.3007510215262401TruSeq Adapter, Index 3 (97% over 34bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG41170.29368879402835163No Hit
CAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC37380.26665258976875844Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT32950.23505090510648982Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32330.23062809596639805No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32000.22827402013376857No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAAATCTCGTATGCCGTC31430.22420788915013581RNA PCR Primer, Index 17 (97% over 34bp)
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26550.18939610107973612No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT24010.17127685073161822No Hit
CAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA23220.1656413358595658Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA19610.13988917296322506Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC19060.1359657132421759Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA17650.12590738923003172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT17230.12291129271577601No Hit
CAAAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG16940.12084255940831375Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC15500.11057022850229416Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15320.10928618713904172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG15290.10907218024516631No Hit
AAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG14720.10500604926153355Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14600.10415002168603191No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14480.10329399411053027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA14430.10293731595407128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)