FastQCFastQC Report
Sun 26 Apr 2020
563_S59_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename563_S59_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8332688
Sequences flagged as poor quality0
Sequence length100
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA1182441.4190378902942244No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG967141.1606578813463315No Hit
TCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGG400740.4809252428508064No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA340550.40869164908130484No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG328050.3936904873913436No Hit
TCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACC192410.23090988166123583No Hit
CGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGT182070.2185009207112999No Hit
GTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGAC180060.2160887339115541No Hit
CGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGT178500.21421658893264695No Hit
TGTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCC164460.19736728412248242No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA151620.18195809083455422No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT145010.1740254765329027No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG136170.1634166549857621No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT134040.16086045703379268No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA131520.1578362228370965No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT127240.15269982507445376No Hit
TCTTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAA121880.14626732694179836No Hit
GATTGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAG120780.14494722471308177No Hit
TTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTT119540.14345910947343762No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT118830.14260704348944783No Hit
CAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG113200.13585052026428926No Hit
TTGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGCT112740.1352984775140987No Hit
TTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAA112580.13510646264446718No Hit
CGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACCG111920.13431440130723724No Hit
TGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAA108260.12992206116441657No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA102490.12299752492833044No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA101700.1220494515095249No Hit
CAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTAC101440.12173742734637372No Hit
CTTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAA99710.11966126656848307No Hit
GCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTA97430.11692505467623412No Hit
CTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGT95750.11490889854510335No Hit
ATTGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGC95550.11466887995806394No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT93560.11228069501702212No Hit
TTCTTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAA91280.10954448312477318No Hit
CCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAA90760.1089204347984708No Hit
CGGCGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCA90510.10862041156467156No Hit
GGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGCTTT88360.10604021175399823No Hit
GCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAA88180.10582419502566279No Hit
TGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGCTT87150.10458809930240998No Hit
CCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGC86370.10365202681295639No Hit
GCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATA83980.10078380469783581No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)