FastQCFastQC Report
Sun 26 Apr 2020
562_S77_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename562_S77_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16068123
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGGACATCTCGTATGCCGT1096100.6821580840525058TruSeq Adapter, Index 7 (96% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGGACATCTCGTATGCCGTC586660.3651079842990995TruSeq Adapter, Index 7 (96% over 32bp)
TCACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT544710.33900039226734824No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGGACATCGCGTATGCCGT392270.2441293236303954TruSeq Adapter, Index 7 (96% over 33bp)
CACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTG373460.23242291585644448No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGACATCTCGTATGCCG369610.23002686748165918TruSeq Adapter, Index 7 (97% over 34bp)
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA353790.22018128688708694No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC337230.20987516712437415No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA324350.20185929619781975No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG301730.1877817340581722No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG297330.1850433930584176No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT290310.18067449446335454No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA279690.1740651350503105No Hit
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG275680.17156951063917047No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA256030.1593403286743573No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA244370.15208372502500758No Hit
GCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGACTG239770.14922091397980958No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA234750.14609671583918046No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG233120.14508228496881684No Hit
TAAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAA230330.1433459278348815No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT215240.13395466290617764No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT214030.13320161913124515No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGGACATCGCGTATGCCGTC201840.12561516986147042TruSeq Adapter, Index 7 (96% over 32bp)
GAGGAAGAGCACACGTCTGAACTCCAGTCACCAGGACATCTCGTATGCCG199910.12441403392294173TruSeq Adapter, Index 7 (96% over 32bp)
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT191130.11894979892797684No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT179380.11163718375817759No Hit
AGGAAGAGCACACGTCTGAACTCCAGTCACCAGGACATCTCGTATGCCGT175760.10938427593565221TruSeq Adapter, Index 7 (96% over 33bp)
TGGAAGAGCACACGTCTGAACTCCAGTCACCAGGACATCTCGTATGCCGT163380.10167958012270631TruSeq Adapter, Index 7 (96% over 33bp)
GATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGGG162900.10138085201364218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)