FastQCFastQC Report
Sun 26 Apr 2020
561_S69_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename561_S69_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6408963
Sequences flagged as poor quality0
Sequence length100
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA990341.5452421866064758No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG882291.3766501694579918No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG427630.6672374298306918No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA378450.5905011465973512No Hit
TCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGG347030.5414760547065103No Hit
TGTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCC188900.2947434709796265No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT157310.24545312556805213No Hit
CGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGT136870.21356029048693212No Hit
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG134980.210611295462308No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT133750.20869210822406056No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG130040.2029033402127614No Hit
TCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACC128460.2004380427847688No Hit
GTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGAC124950.1949613377390383No Hit
GATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGGG87180.1360282466913914No Hit
CAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG81750.1275557371762015No Hit
AGTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCC79680.1243258854825656No Hit
TACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCT76800.11983217877837647No Hit
GTGAGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA69800.10890997498347237No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT69010.10767732626947606No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT66910.10440066513100481No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA65790.10265311252382016No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA64610.1008119410269649No Hit
CGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACCG64380.10045306861656089No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)