FastQCFastQC Report
Sun 26 Apr 2020
552b_S54_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename552b_S54_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4369784
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT80450.18410520977695924No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT66640.15250181702345014No Hit
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG63810.1460255243737448No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA59310.13572753252792358No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG55730.1275349079039147No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG54030.12364455542882669No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA53120.1215620726333384No Hit
TTGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGCT46390.10616085371725469No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT45550.10423856190603471No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT44600.10206454140525023No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC44440.10169839058406548No Hit
TGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGCTT43870.1003939782835948No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)