FastQCFastQC Report
Sun 26 Apr 2020
542_S3_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename542_S3_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6287099
Sequences flagged as poor quality0
Sequence length100
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA419440.6671439403133305No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG382950.6091044534212043No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG182830.29080184676589316No Hit
TCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGG162890.2590861063266222No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA161210.2564139677138852No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT152520.24259201262776361No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT129870.2065658581167562No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA129210.20551608937603816No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT121780.19369823824946927No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA119580.19019900911374227No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA114340.18186448153591983No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT113220.18008305579409517No Hit
TTGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGCT106500.1693945013431473No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT103910.1652749543151778No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA102320.162745965985266No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA101570.16155304696172273No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG99610.1584355519135296No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC96920.15415694901575433No Hit
TGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGCTT95760.15231190092600738No Hit
TGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAA92910.14777880863654286No Hit
TGTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCC89740.1427367375636999No Hit
TTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAA89460.14229138112824374No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT87850.1397305816243708No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA83880.13341606359308164No Hit
TACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCT81290.12929651656511215No Hit
GGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGCTTT81200.12915336628228696No Hit
GCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATA79760.12686296175708384No Hit
CATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAG79630.12665618912633633No Hit
CAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG77490.12325239351249281No Hit
ACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTA77300.12295018735986184No Hit
TCTTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAA75260.11970544761582408No Hit
CGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGT73860.11747866543854327No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG69680.110830130080662No Hit
ATTGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGC67660.1076172015105854No Hit
AAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTG67410.10721956183607098No Hit
TACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTT66990.10655152718288673No Hit
AAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGA65280.10383167180920802No Hit
GCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAA64070.10190709578455819No Hit
CGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGT63690.10130268347929626No Hit
CCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAA63290.10066646000007316No Hit
CTTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAA63220.10055512089120913No Hit
GATTGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAG63080.10033244267348103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)