FastQCFastQC Report
Sun 26 Apr 2020
531_S44_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename531_S44_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5086934
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT75690.14879296645091128No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG59980.11790992373795295No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT57430.11289708103152114No Hit
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA55250.10861159197268924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)