FastQCFastQC Report
Sun 26 Apr 2020
529_S53_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename529_S53_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6972407
Sequences flagged as poor quality0
Sequence length100
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA392680.5631914488067034No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG349480.5012329314682863No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG303840.43577490528019946No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA293700.4212318644049322No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT170410.24440627175091756No Hit
TCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGG146610.21027171821725266No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG131090.188012547173451No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA122500.17569255495268707No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC113490.16277018825779965No Hit
TCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACC110180.1580229037117311No Hit
GTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGAC106450.1526732446915391No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA106190.15230034620755786No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA105880.1518557364766572No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT93490.134085689490014No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA89720.1286786614722864No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA88810.12737351677835215No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT80650.11567024128109561No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT80560.11554116103664057No Hit
CAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG79650.11423601634270633No Hit
TTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAA78090.11199862543881905No Hit
AAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTG76790.11013413301891298No Hit
TCTTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAA73100.10484184299625653No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG72160.1034936715541706No Hit
TGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAA70370.10092640891445379No Hit
TGTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCC70160.10062522167739205No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG69890.10023798094402694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)