FastQCFastQC Report
Sun 26 Apr 2020
528_S27_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename528_S27_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8784739
Sequences flagged as poor quality0
Sequence length100
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG186960.2128236251526653No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA148100.16858782030974398No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT130490.14854169258756578No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT115450.13142109287481393No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA114810.13069255671682448No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC107690.12258759195919194No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG99150.11286618760102037No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA96600.10996342634653118No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT93280.10618414502696095No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA92350.10512549092238255No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT91460.10411237032767848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)