FastQCFastQC Report
Sun 26 Apr 2020
523_S4_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename523_S4_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7083612
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA266650.3764322495359712No Hit
CCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAA168810.2383106245796636No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT102660.144926063144057No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA97220.13724636527240622No Hit
CAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAAGGGCAGG94700.1336888581700974No Hit
TCAATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCG94590.13353357016166328No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT94520.1334347505199325No Hit
CAATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGG90790.1281690753248484No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT90340.1275338061994361No Hit
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG86290.12181638407072551No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA82550.11653659178396558No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG81390.11489900914956946No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG75810.10702167199445707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)