FastQCFastQC Report
Sun 26 Apr 2020
521_S65_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename521_S65_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11767309
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA503190.4276168833503055No Hit
TCACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT487390.4141898542818923No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC474700.40340574042884403No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG461540.39222221495160875No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT370460.31482134105597126No Hit
TAAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAA355520.3021251502786236No Hit
CACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTG354350.3011308702779879No Hit
CGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGT343980.29231832018688386No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA334910.2846105256520416No Hit
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA285610.24271479571072707No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG274090.2329249618583144No Hit
CTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGT260550.22141850783386416No Hit
TTAAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGA252310.21441605723109677No Hit
GCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAG250390.21278441825569464No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT247470.2103029673139373No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG237110.20149891534249675No Hit
GCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGACTG229230.19480239704761726No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA214830.18256510473210144No Hit
AAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTG212790.18083148832073673No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA206470.17546067669337143No Hit
TCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAAAA191600.16282397275366867No Hit
GCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTA189030.16063995600013564No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA187800.15959468728151865No Hit
AAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGA175900.149481924881891No Hit
CAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTAC163740.139148211371011No Hit
TCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACC155460.1321117682895894No Hit
TAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC154550.13133843940020612No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA153870.13076056726308455No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT145640.1237666147799807No Hit
GTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAAA136980.11640724315134413No Hit
CTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGAC133230.11322044827751189No Hit
CAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG132420.11253210058476411No Hit
CTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAAC129780.11028859699358622No Hit
AAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGG129540.11008464212166096No Hit
CATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAG128490.10919233955698793No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA119520.10156952621878121No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)