FastQCFastQC Report
Sun 26 Apr 2020
506_S47_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506_S47_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5879831
Sequences flagged as poor quality0
Sequence length100
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA344140.5852889309233548No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG284780.48433364836506354No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG283190.4816294890108236No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT252450.42934907482885143No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA238970.40642324583818823No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG208580.3547380868599795No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA159790.2717595114553462No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA152290.25900404280327105No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG136330.231860405511655No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT120980.20575421300374108No Hit
TCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGG119140.20262487136109864No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA113140.19242049643943848No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA113050.19226743081561357No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC112930.19206334331718036No Hit
AAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGA109900.18691013398174197No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA108480.18449509858361574No Hit
AAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTG104470.17767517467763955No Hit
GTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGAC96590.16427342894719252No Hit
TCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACC94780.16119510917915839No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT89920.15292956549261366No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT83850.1426061395302008No Hit
TTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAA82090.13961285621984712No Hit
TGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAA80610.13709577707250428No Hit
AAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGG77440.13170446565556052No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT75320.12809891984990726No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA74220.1262281177809362No Hit
TCTTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAA73500.12500359279033701No Hit
CGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGT73460.12493556362419261No Hit
CAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG71740.12201030947998336No Hit
TAAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAA70120.11925512825113511No Hit
CTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGT69350.1179455668028554No Hit
GCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAG65700.1117379053921788No Hit
TCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACT62780.1067717762636375No Hit
GCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTA62170.1057343314799354No Hit
CGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACCG61370.10437374815704736No Hit
ATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGAT61020.10377849295328387No Hit
GTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAAA59060.10044506381220822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)