FastQCFastQC Report
Sun 26 Apr 2020
492_S40_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename492_S40_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7972788
Sequences flagged as poor quality0
Sequence length100
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA201900.25323638355867484No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG191720.24046795173783628No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA170810.21424124158324542No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG168990.2119584767586947No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA129500.16242749713149277No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT128920.16170002262696562No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT128630.16133628537470204No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA107490.13482109395107458No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG106510.13359191289170114No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA103070.12927723652002285No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA96420.12093636504570296No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA90200.11313482811784283No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT89710.11252023758815612No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC86470.108456414493901No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT83600.10485666996287873No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)