FastQCFastQC Report
Sun 26 Apr 2020
488_S26_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename488_S26_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6926901
Sequences flagged as poor quality0
Sequence length100
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA169700.2449868996250993No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG169630.24488584433356272No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT129690.18722658227683636No Hit
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA128120.18496005645237315No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG117460.16957077919837457No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG111750.16132755470303387No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA104800.15129420790047382No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA93100.1344035377436461No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG83970.1212230404332327No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC74710.10785486900996563No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT73530.10615136552406335No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA73240.10573270788769755No Hit
TCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACC70390.10161831387513695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)