FastQCFastQC Report
Sun 26 Apr 2020
484_S43_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename484_S43_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5194506
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA105980.20402325071912517No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT83070.15991896053253188No Hit
CCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAA67230.1294252042446385No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT66930.1288476709816102No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA61120.11766277678762907No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA57030.10978907330167682No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG55920.10765220022847216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)