FastQCFastQC Report
Sun 26 Apr 2020
472b_S48_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename472b_S48_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6292167
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG94940.15088601430953755No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT82540.13117897220464744No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT76780.1220247332914082No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA73780.11725690052409607No Hit
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA68020.1081026616108568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)