FastQCFastQC Report
Sun 26 Apr 2020
462b_S64_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename462b_S64_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10172164
Sequences flagged as poor quality0
Sequence length100
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG252920.2486393259094132No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT229530.22564520194523013No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG203190.19975100676709495No Hit
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG201060.19765705704312278No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA174530.17157607761730934No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA172100.16918720539700305No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA166150.1633379092197098No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA157550.15488346432479852No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA141380.13898714177238983No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC140370.13799423603473165No Hit
GATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGGG134380.13210561685792718No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT132550.1303065896302891No Hit
AAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTG130700.12848790090289539No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA129210.12702311917110262No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT126240.1241033864573949No Hit
CATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAG120770.11872596627423625No Hit
TCACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT118970.11695643129623157No Hit
GCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATA115020.11307328509449907No Hit
GGGCATGGGGGGTATATAAAGACCCTTCTAACATTATGTCTGATATTGAA111700.10980947613506821No Hit
GTGGGCATGGGGGGTATATAAAGACCCTTCTAACATTATGTCTGATATTG107380.10556259218785698No Hit
AAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGA106530.10472697844824366No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT104480.10271167472329387No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT102350.10061772499932167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)