FastQCFastQC Report
Sun 26 Apr 2020
461b_S31_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename461b_S31_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4635478
Sequences flagged as poor quality0
Sequence length100
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG71970.15525906929123598No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT69060.1489813995449876No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG59450.12824998845857968No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT55560.11985818938197959No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT50450.10883451501657435No Hit
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA49530.10684982217583601No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA46440.10018384296074752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)