FastQCFastQC Report
Sun 26 Apr 2020
45_S63_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename45_S63_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7231064
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA134750.18634878629203117No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT100000.1382922347250695No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA94540.1307414787090807No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT89140.12327369803392695No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT81660.11292943887649175No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT81130.11219649003244889No Hit
CGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGT76580.10590419335245822No Hit
GAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAAA75670.10464573401646009No Hit
CGAATGGGGCAACCCAGCTGGCTGAAGGCCAGTTACCCGAGAGGGATAAA75000.10371917604380214No Hit
ACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTA74330.10279261807114416No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)