FastQCFastQC Report
Sun 26 Apr 2020
452b_S2_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename452b_S2_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5550931
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA82860.14927225721234869No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT71350.12853699676684865No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG62630.11282792021734733No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA59760.10765761635300457No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)