FastQCFastQC Report
Sun 26 Apr 2020
44_S71_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename44_S71_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365492
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCACAATCTCGTATGCCGT51410.3764943331780779TruSeq Adapter, Index 6 (97% over 34bp)
CAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC32930.24115849818234011Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT30280.22175157379171756Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACAATCTCGTATGCCG29140.21340293461990256TruSeq Adapter, Index 6 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCACAATCTCGTATGCCGG25440.18630647414997673TruSeq Adapter, Index 6 (97% over 34bp)
CAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA21930.16060145354202002Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC20360.14910376626153796Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA19640.1438309415214443Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG18410.13482319925711758Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCACAATCGCGTATGCCGT16750.1226664088841238TruSeq Adapter, Index 6 (97% over 34bp)
AAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC16300.11937089342156526TruSeq Adapter, Index 5 (97% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCACAATCTCGTATGCCGTC15090.11050961851113004TruSeq Adapter, Index 6 (96% over 33bp)
AAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC14480.10604236421743957TruSeq Adapter, Index 6 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)