FastQCFastQC Report
Sun 26 Apr 2020
444_S34_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename444_S34_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8059379
Sequences flagged as poor quality0
Sequence length100
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA294820.36580982232005715No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG265240.32910724262998425No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA242760.3012142746978396No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG237090.29417899319538143No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT160880.19961835769232344No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG160300.19889869926702788No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA149980.18609374245832094No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA131790.16352376529258644No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC116810.14493672527374626No Hit
TCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGG116420.14445281702225443No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA113360.14065599843362622No Hit
TCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACC108220.13427833583704155No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG104230.12932758218716356No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT102850.12761529145111553No Hit
GTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGAC99620.1236075384964524No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT85260.10578978851844541No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA84520.10487160363099936No Hit
TCTTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAA82070.10183166717931989No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG80970.10046679775203524No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)