FastQCFastQC Report
Sun 26 Apr 2020
443_S36_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename443_S36_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6168835
Sequences flagged as poor quality0
Sequence length100
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA136960.22201923053542524No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG131300.2128440783389408No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG121100.196309351765771No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA111150.18017988809880633No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA99640.16152158389712157No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT99580.16142432079963234No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG87860.14242559575673525No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT86680.140512754839447No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT77830.12616644795978496No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT77330.12535592214737468No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA74590.12091424069536631No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA74290.12042792520792014No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT72680.11781803209195901No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT64690.10486582960964268No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA63220.10248288372115641No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)