FastQCFastQC Report
Sun 26 Apr 2020
442b_S60_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename442b_S60_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5438425
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT259360.47690277975700684No Hit
CACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTG179590.3302242836850743No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG129800.23867204199745332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA127510.23446126406082643No Hit
GCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGACTG122510.22526742577124811No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT108280.19910176199910817No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA95910.17635620607069144No Hit
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA88210.1621976951047408No Hit
CTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGT84700.15574362062545682No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC81220.1493447091759103No Hit
GCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAG80700.14838854999379417No Hit
TCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAAAA80180.14743239081167803No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA73510.13516781053338053No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA72370.13307161540335669No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG70350.12935730473436705No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA65410.12027379250426364No Hit
CTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGAC64770.11909698120319762No Hit
CGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGT60320.1109144651254729No Hit
AAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTG59050.10857923019992001No Hit
TAAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAA57480.10569236497699243No Hit
AAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGA56630.1041294124677641No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG54660.10050704018167024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)