FastQCFastQC Report
Sun 26 Apr 2020
441_S73_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename441_S73_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9183978
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCGT2403822.6174060957027554TruSeq Adapter, Index 7 (96% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCGTC1755661.9116552761777088TruSeq Adapter, Index 7 (96% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCGCGTATGCCGT794940.8655726309448911TruSeq Adapter, Index 7 (96% over 32bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG719740.7836909016985886TruSeq Adapter, Index 7 (96% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCGCGTATGCCGTC568300.6187950363121515TruSeq Adapter, Index 7 (96% over 31bp)
GAGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG555300.6046399501392533TruSeq Adapter, Index 7 (96% over 31bp)
AGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCGT498320.5425971185906586TruSeq Adapter, Index 9 (96% over 32bp)
TGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCGT469590.511314378148554TruSeq Adapter, Index 9 (96% over 32bp)
GGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCGT297910.32438013244369707TruSeq Adapter, Index 9 (96% over 32bp)
TTGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG258190.28113089992158086TruSeq Adapter, Index 9 (96% over 33bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCGCGTATGCCG243430.26505943285142886TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGC220700.2403098091045079TruSeq Adapter, Index 7 (97% over 35bp)
GCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG213350.2323067411529078TruSeq Adapter, Index 9 (96% over 33bp)
CGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCGTC198960.2166381496122922TruSeq Adapter, Index 9 (96% over 31bp)
ACGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG180770.1968319174980602TruSeq Adapter, Index 9 (96% over 33bp)
GGAGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCC161510.17586061290652047TruSeq Adapter, Index 9 (96% over 32bp)
GAGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCGCGTATGCCG160080.17430355342750167TruSeq Adapter, Index 7 (96% over 31bp)
TGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCGTC155680.16951260118436695TruSeq Adapter, Index 9 (96% over 31bp)
AGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCGCGTATGCCGT155350.16915327976613184TruSeq Adapter, Index 9 (96% over 32bp)
CCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG153080.16668158394978735TruSeq Adapter, Index 9 (96% over 33bp)
TAGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG151600.165070081831642TruSeq Adapter, Index 9 (96% over 33bp)
CGGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG149290.1625548319039963TruSeq Adapter, Index 7 (96% over 31bp)
AAGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG148550.16174908084492362TruSeq Adapter, Index 7 (96% over 31bp)
TGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCGCGTATGCCGT141520.15409444578373335TruSeq Adapter, Index 9 (96% over 32bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGC137970.15022901840574965TruSeq Adapter, Index 9 (97% over 35bp)
AGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCGTC130590.1421932848706737TruSeq Adapter, Index 9 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCC127650.13899205769003367TruSeq Adapter, Index 7 (97% over 34bp)
AGAGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCC116110.12642669657963032TruSeq Adapter, Index 7 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATG114410.12457564684932826TruSeq Adapter, Index 7 (97% over 35bp)
TCGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCGT108440.11807519573762044TruSeq Adapter, Index 7 (96% over 30bp)
CAGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG108030.11762876609678288TruSeq Adapter, Index 7 (96% over 31bp)
CAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC106390.11584304753343268Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT99070.10787264516530855Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCCG99060.10786175663748322TruSeq Adapter, Index 7 (96% over 31bp)
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT98700.1074697696357722No Hit
ATTGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCC96940.10555338873851831TruSeq Adapter, Index 9 (97% over 34bp)
AGCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGATCTCGTATGCC93670.1019928401396432TruSeq Adapter, Index 9 (97% over 34bp)
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG92840.10108909233014277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)